Annexin V and NaN3
Keith Bahjat (kbahjat@ufl.edu)
Wed, 15 Oct 1997 21:51:11 -0400
I am hoping to look at apoptosis within a specific TCR
population (VB8) following superantigen stimulation, and
plan to use one of the handy Annexin V kits for this. I want
to add sodium azide to the buffer to keep my TCR from
capping. Will this interfere with the Annexin V binding?
The only reason I ask is that EVERY buffer for cell
preparation that I've used has sodium azide, and the
omission of this from the CaCl buffer in the annexin kits is
suspicious.
All insights (and wild guesses) appreciated!
kb
--
Keith Bahjat
Graduate Assistant
University of Florida
College of Medicine
Gainesville, Florida
kbahjat@ufl.edu