apoptosis

Mayumi Naramura (MNARAMURA@atlas.niaid.nih.gov)
Sun, 2 Nov 1997 13:20:33 -0500

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I'm trying to study apoptosis of
1. fresh mouse thymocytes induced by
2. TCR stimulation using
3. flow cytometry, either by
3a. Annexin V or by
3b. TUNEL.
I know there are tremendous amount of literature related to this, but so
far I have not come across with ones that contain all the "keywords" I
listed above. Specifically, I found most of the study has been done
using "immature" T cell lines or hybridomas instead of "fresh mouse
thymocytes".
I tried it a couple of times, but I cannot see any difference between
cells stimulated with anti-CD3 and non stimulated control because a
significant portion of cells are Annexin V (+) in the control. Any
suggestions?
Mayumi Naramura, M.D.
NIH/NIAID
Lab of Immunology
Twinbrook II, Rm. 125
12441 Parklawn Drive
Rockville, MD 20852
Phone: 301-402-4595
FAX: 301-594-2522
e-mail:mnaramura@atlas.niaid.nih.gov

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