Lipid staining

Keith Bahjat (kbahjat@ufl.edu)
Wed, 12 Nov 1997 21:51:01 -0500

I am attempting to stain neutral lipid bodies within dendritic cells for
the purpose of enumeration (per cell, as well as cells containing
inclusions) and am having trouble finding an adequate procedure.

I have tried Nile Red with and without glutaraldehyde fixation of the
cells, but the background is too high to delineate positive from
negative.

My hope is to find a narrow bandwdth dye that will allow simulataneous
detection of typical fluorochromes, but I'll take what I can get.

Any suggestions appreciated.

Thanks.

--
Keith Bahjat
Graduate Assistant
University of Florida
College of Medicine
Gainesville, Florida
kbahjat@ufl.edu