RE: CCR5 MAB staining;
I would like to know if anyone has experience staining HOS (transfected with
CCR5 gene) cells or stimulated PBMC's (PHA/IL2) using R&D systems CCR5 clones.
We have tried these clones with various fixing conditions and have not been
successful. If anyone has a protocol for these clones I would like to try it.
Thanks
Stephen P. Perfetto, MS.,MT. (ASCP)
Walter Reed Army Institute of Research
Department of Diagnostic Retrovirology
1600 East Gude Drive
Rockville, MD. 20850