I am attempting to stain neutral lipid bodies within dendritic cells for
the purpose of enumeration (per cell, as well as cells containing
inclusions) and am having trouble finding an adequate procedure.
I have tried Nile Red with and without glutaraldehyde fixation of the
cells, but the background is too high to delineate positive from
negative.
My hope is to find a narrow bandwdth dye that will allow simulataneous
detection of typical fluorochromes, but I'll take what I can get.
Any suggestions appreciated.
Thanks.
-- Keith Bahjat Graduate Assistant University of Florida College of Medicine Gainesville, Florida kbahjat@ufl.edu