Re: Fibroblast??

Richard L. Darley (Darley@cardiff.ac.uk)
Fri, 14 Nov 1997 09:47:58 GMT

>What is a good marker specific for phenotyping fibroblast from
>cultures.?

Jim,
I'm glad you asked this one. I posed a similar question a few weeks
ago and the only replies I got were "...if you find a good marker let
me know".

I have been reccomended by a pathologist to try vimentin
(intermediate filament). Since this is intracellular, it is useless
for my application so I have not tried it.

You don't say what species your fibroblasts are or what cells you are
trying to distinguish them from. If they are different species you
could try a species-specific marker (eg MHC class I)

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From: Dr Richard Darley, Ph.D.
LRF Differentiation Unit
Department of Haematology (A7)
University of Wales College of Medicine
Heath Park
Cardiff, CF4 4XN
U.K.
tel (+44) 12 22 74 34 85
or (+44) 12 22 74 45 24
Fax (+44) 12 22 74 45 23
e-mail: darley@cf.ac.uk