We use a kappa/lambda/CD19 triple to establish clonality for B cells,
but we are noticing that some of our cases of Hairy cell leukaemia
appear "double stained" for kappa and lambda. We use 2% bovine serum
albumin in our wash buffer and have tried blocking with various
reagents, but no luck so far. Has anyone come across this problem, or
have any ideas?
Thanks in advance
Steve Couzens
(Couzens@cf.ac.uk)