BrdU-Proliferation assay question

Andreas Schroeder (h82@ix.urz.uni-heidelberg.de)
Thu, 26 Sep 1996 17:51:24 +0200

Is anybody of you aware of a good (means working) protocol for
a Br-desoxyuridine-FACS-procedure? I have used the B-D-protocol
provided in their monoclonal antibody resource book, but without
success :-(
In particular I am looking for information about the FACS-setup
for aquisition and analysis of cell proliferation and hints
about possible pitfalls in the procedure.

Any help is greatly appreciated.

--
Andreas Schr=F6der