Dennis
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* Dennis J. Young Voice : (619) 822-0407 *
* Flow Cytometry Core Facility FAX : (619) 822-0412 *
* University of California, San Diego USA e-mail: djyoung@ucsd.edu *
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______________________________ Reply Separator _________________________________
Subject: Re: PE/Cy5 on a FACS Calibur
Author: steven_micko@email.eushc.org at @UCSD
Date: 9/25/96 11:03 AM
Calman,
We are a beta site for the second (red) laser on a FACSCalibur. I do my
daily QC with Calibrites (FTC, PE, blanks, PerCP and APC), and it works
fine. Our biological work uses PerCP, APC, and Cychrome, and I have had
no complaints. I don't know about PE/Cy5; is it the same as CyChrome?
The present status of 4 color work on the Calibur is that it is in Beta
testing. We use cross-beam compensationS between FL1 & FL2, FL2 & FL3,
FL3 & FL4.
Steve Micko
Emory Hospital
Atlanta
______________________________ Reply Separator _________________________________
An associate has asked me a third hand question regarding the utility of
using PE/Cy5 conjugates on a FACScaliber in 3 color applications.
Questions for the group and for BD lurkers in particular:
1. Do PE/Cy5 conjugates work any better or worse on a Calibur than on a
FACSCAN in 3 color/ 1 laser applications.
2. What is the present status of 4 color work on the Calibur? Any plans
for cross-beam compensation to allow simultaneous use of FITC, PE,
PE/Cy5 and APC?
Thanks,
Calman Prussin
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>Subject: Re: PE/Cy5 on a FACS Calibur