In a tube which combines CD2(FITC)/CD4(PE)/CD8(PerCP) mAb's, which
values are most appropriate to use to calculate the CD4:CD8 ratios
correctly. Would one use:
(i) the total %CD4 and the total %CD8 derived from single parameter
histograms?
or
(ii) the %CD4+CD8- and the %CD8+CD4- derived from the CD4 vs CD8 dot
plot quadrants?
or
(iii) Should one be using the %CD2+CD4+ and the %CD2+CD8+ values
(Something that was just recently suggested to me)?
Thanks for all replies/insights in advance.
Diane Ladouceur
(613-957-0998)
NB/ We were using CD2 instead of CD3, as this work is being done on
Cynomolgus monkeys, and at the time we had not yet identified a CD3 mAb
which cross-reacted with this species.