Re: saturation binding & fluorescence intensity

Thomas Delohery (t-delohery@ski.mskcc.org)
Wed, 16 Oct 1996 16:39:44 +0530

>This is not true... fluorescence intensity is ALWAYS proportional to the
>antigen
>density. The problem at sub-saturating amounts of antibody is that it is also
>proportional (roughly) to antibody concentration as well as the time of the
>stain! We have done quantitative antigen density measurements with antibodies
>at subsaturating concentrations. However, to do so, we needed to ensure that
>(1) the staining volume was precisely controlled; (2) the amount of
>antibody was
>precisely controlled; and (3) the timing of the incubation (and volume of
>subsequent washes, for that matter) was controlled.
>
>No arguments here, Tom, just the facts.
>
>mr

Thanks for the clarification. BTW, that wasn't the part of my post where I
expected an argument.

td

--
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 Thomas Delohery                        | Internet: t-delohery@ski.mskcc.org
 Manager, Flow Cytometry Core Facility  |    Phone: (212) 639-8729
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