Jim Weaver wrote:
> Under most circumstances the assumption of linearity will be
>reasonable, however we need to be aware of the occasional
>circumstances where the assumption will not be correct.
Since immunofluorescence intensity is generally proportional to Ag density
if one carefully controls the staining conditions - what is the
proportionality?
Unless otherwise stated, proportional implies a direct or linear relationship.
This would hold for MAbs directly labeled with a single fluorochrome (FITC),
but what about phycobiliproteins? Or streptavidin conjugates? For a MAb
conjugated to PE wouldn't the relationship of fluorescence to Ag density be
geometric?
td
-- ============================================================================== Thomas Delohery | Internet: t-delohery@ski.mskcc.org Manager, Flow Cytometry Core Facility | Phone: (212) 639-8729 Memorial Sloan-Kettering Cancer Center | Fax: (212) 794-4019 1275 York Ave. Box 98 | New York, NY 10021 | ==============================================================================