When considering only the filter sets that are shipped with an instrument,
when performing 4 color experiments on the Coulter XL and the BD
FACSCalibur, wouldn't a greater signal to noise ratio be achieved on the
FACSCalibur due to the increased "width" of the bandpass filters??
In other words: As Coulter has managed to achieve four color capabilities
from a single 488 nm laser, they have in turn used smaller bandwidths (?)
in the filters to the 4 PMTS, such that each filter only has about a 15 nm
range. Does this not decrease the signal to noise ratio obtainable with
this filter set for all fluorescent channels?
Would I be better off when performing 3 color experiments on my XL to use
different filters to maximize my signal to noise ratio?
Any help appreciated.
A curious guy,
Keith Bahjat
Kbahjat@nwu.edu