Actually, Skip Maino from BD has described a antigen specific proliferation
test that works in HIV. Patient's PBMC are cultured with and without CMV
extract. After culture the cells can be measured for CD69. They showed that a
CMV+ donor would have 10%+ CD69 cells while a CMV sero negative donor would have
only 3% CD69 cells. This might be a better approach than quantitating the
actual AG on PBMC.
Stephen P. Perfetto
Department of HIV Disease Prevention
Walter Reed Army Institute of Research
1600 East Gude Drive
Rockville, MD. 20850
_______________________________________________________________________________
Subject: CMV-specific surface antigens
From: "Gregor Rothe" <gregor.rothe@klinik.uni-regensburg.de> at
Internet_Gateway
Date: 11/8/96 2:23 PM
Hello to all,
Is there anyone on the list with experience in the flow
cytometric analysis of CMV-specific nuclear or cell-
surface antigens on peripheral blood leukocytes?
Any recommendations regarding reagents?
Thanks for any help,
Gregor Rothe
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Institute for Clinical Chemistry and Laboratory Medicine
University of Regensburg
D-93042 Regensburg, Germany
Tel. +49 (941) 944-6204
Fax +49 (941) 944-6202
Internet: Gregor.Rothe@klinik.uni-regensburg.de
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