Re: Krypton Laser for GFP?

SPERFETTO (sperfetto@hiv.hjf.org)
Thu, 14 Nov 1996 09:40:20 -0500

All of our work on GFP is currently using the human gene form in transfected
cells which can easily seen on the Fl. microscope and excited by the 488nm laser
line. Most of these cells have Fl.intensities in the 3rd decade! I think your
approach should start with the Fl microscope and examine the mutant form in
these cells. Your data seems to indicate a problem with getting the GPF gene
into your cell system.

Stephen P. Perfetto
Walter Reed Army Institute of Research
1600 East Gude Drive
Rockville, MD. 20850
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Subject: Krypton Laser for GFP?
From: Pizzo@nso1.uchc.edu (Pizzo;Eugene) at Internet_Gateway
Date: 11/13/96 7:33 AM

All Colleagues,

A researcher here at UCONN is considering using the 413nm violet line
of a Krypton laser to excite mutant GFP. This researcher has been
somewhat dissatisfied with our results using 488nm excitation on the
FACStar Plus (by the way we've been using a 500 EFLP in Fl1 to
capture the emission). Can anyone enlighten me as to the worthiness
of this approach? Is there a new GFP out there or is 413nm excitation
really significantly better?

Gene Pizzo/UCONN Health