>>>It would appear the 'Calibur is able to directly detect APC, Cy5 or Cy7
>>>as the 4th color. In that case the use of ANY of the PE tandems may
>>>present some problems with the second laser. Of course this isn't a
>>>problem if you use PerCP for your "near red" (FL3).
I don't think the 635 nm laser will effectively excite Cy7 directly, and even if
it does the stock 661/16 in FL4 will not pass it's emission. We are certainly
able to detect APC and Cy5 in the FL4 channel.