Use calibrated beads: add a known concentration of beads to all samples and
calculate the ratio of WBC:Beads. (BDIS has a product called TrueCOUNT - tubes
with beads.
Dennis
*************************************************************************
* Dennis J. Young Voice : (619) 822-0407 *
* Flow Cytometry Core Facility FAX : (619) 822-0412 *
* University of California, San Diego USA e-mail: djyoung@ucsd.edu *
* http://www-core.ucsd.edu/flow-core.html *
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______________________________ Reply Separator _________________________________
Subject: Re: Absolute counts from flow cytometry
Author: douglas@HELIX.NIH.GOV at @UCSD
Date: 11/15/96 8:38 AM
At 04:29 PM 11/14/96 EST, you wrote:
>
>We are trying to generate absolute counts for different cell types in the
>peripheral blood of mice. What are your preferred methods? ......
Jeff Carrell
Human Genome Sciences
Jeff,
You may want to look at LDS-751. It is a nucleic acid dye not requiring
permeabilization and can be used in whole blood with no lysing.
See the following reference:
Multidimensional Flow Cytometric Blood Cell Differentiation without
erythrocyte lysing, Terstappen, et al. in Blood Cells (1991) 17:585-602.
The dye is available from Exciton, Inc (513) 258-3937, Dayton, Ohio.
Good luck.
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>Date: Fri, 15 Nov 1996 08:38:36 -0400
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>From: Doug Terle <douglas@HELIX.NIH.GOV>
>Subject: Re: Absolute counts from flow cytometry