I have a user who uses FDG (b-gal) and a surface marker to label some cell
spleen cells. Because would not like to stay until late at night, I'm
looking for a method to keep the cells overnight and analyse them the next
day.
Therefore my questions:
1. Is it possible to fix the cells after the FDG reaction (formaldehyd 2%)?
2. If you keep the cells without fixation at 4° ovn can you still analyse
them on the FACS the next day, or is the FDG product not very stable ?
Any experience on this topic would avoid me to work until late at night....
Thank you very much, and have a nice weekend.
Matthias
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Matthias Haury _/_/_/ _/_/_/ _/_/_/
Flowcytometry - Immunology _/ _/ _/ _/ _/
Institut Pasteur Paris _/ _/_/_/ _/_/_/
Email: mhaury@pasteur.fr _/ _/ _/
Tel: 33 (01) 40 61 31 29 _/_/_/ _/ _/
Fax: 33 (01) 45 68 86 39 INSTITUT PASTEUR PARIS
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