The PerCP and PE-Cy5 will work on the FACScan very well. Both have the
emission maxima around 675nm (677 for PerCP and very close for PE-Cy5, but I
do not remember exact value). You only have to know what kind of a filter do
you have for the collection of the FL3 signals. 650LP as far as I remember
was the standard in FACScan. It will work all right. As you probably know
PE-Cy5 travels under so many aliases one may easily get confused. DAKO,
Serotec, Immunotech etc call it simply PE-Cy5, PharMingen calls it
'Cy-Chrome', Caltag prefears 'TriColor', Sigma for a change likes 'Quantum
Red', and I suspect Exalpha's 'AlphaRed' is the one too.
As far as I know there is no commercial source of anti-human or anti-mouse
immunoglobulins directly conjugated to PE-Cy5 or PerCP. There are ways to
bypass it, though.
1. Get the biotinylated antibody and the streptavidin conjugated PerCP from
Becton Dickinson, or streptavidin-PE-Cy5 from, say DAKO. The best source of
biotinylated goat anti -human/-mouse MoAbs is Jackson ImmunoResearch.
2.Get those anti-immunoglobulin MoAbs conjugated to FITC or PE and the other
ones you want to use (in a 3 color setup) conjugated to PerCP or PE-Cy5.
This idea does not work if you need ALL of your MoAbs (FL1,2,3) directed
against immunoglobulins, of course.
Hope this helps a bit,
Janek
--------------------------
mmbrozek@cyf-kr.edu.pl
http://www.cyf-kr.edu.pl/~mmbrozek