ANTI-MOUSE CD140a (PDGF Receptor alpha chain), CLONE APA5

SPECIFICITY

The APA5 antibody reacts with Platelet-Derived Growth Factor (PDGF) Receptor alpha chain (CD140, PDGFR-alpha), a receptor tyrosine kinase which is widely expressed on cells of mesenchymal origin in the embryo and adult and on several other cell types during embryonic development, but not on hematopoietic cells.(1,2) PDGFR-alpha binds to PDGF A and B chains, in contrast to PDGFR-beta, which binds only to the PDGF B chain.(3) Biologically active PDGF is a disulphide-linked dimer, forming the AA, AB, and BB isoforms. Binding to PDGF Receptor induces the formation of receptor dimers (alpha alpha, alpha beta, or beta beta), autophosphorylation, and internalization.(3) The mAb APA5 has been demonstrated to block binding of PDGF-AA to PDGFR-alpha-expressing cells in vitro (1) and to block some PDGF-mediated developmental events in vivo.(1,2)

USAGE

This antibody has been tested by immunofluorescent staining (< 1 µg/million cells) with flow cytometric analysis to assure specificity and reactivity. Other reported applications include western blot analysis,(1) ELISA,(1) immunohistochemical staining of acetone-fixed frozen sections(4) and polyester wax-embedded sections,(1) inhibition of ligand binding,(1) and in vivo blocking of PDGF action.(1,2) Since applications vary, each investigator must determine dilutions appropriate for individual use.

Cat. No. Description Clone Isotype Size
28011D Purified anti-mouse CD140a APA5 Rat IgG2a 0.5mg

For Research Use Only. Not For Diagnostic or Therapeutic Use.

REFERENCES

  1. Takakura, N., H. Yoshida, T. Kunisada, S. Nishikawa, and S.-I. Nishikawa. 1996. Involvement of platelet-derived growth factor receptor-a in hair canal formation. J. Invest. Dermatol. 107: 770 - 777.
  2. Fruttiger, M., A.R. Calver, W.H. Krüger, H.S. Mudhar, D. Michalovich, N. Takakura, S.I. Nishikawa, and W.D. Richardson. 1996. PDGF mediates a neuron-astrocyte interaction in the developing retina. Neuron 17: 1117 - 1131.
  3. Heldin, C.-H. 1992. Structural and functional studies on platelet-derived growth factor. EMBO J . 11: 4251 - 4259.
  4. PharMingen. Unpublished results.

PharMingen offers a complete line of antibodies to mouse cell surface antigens and cytokines, including:

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