BACKGROUND
The 10/78 antibody reacts with NKR-P1A, a 60-kDa homodimer expressed on all natural killer (NK) cells and a small subset of T lymphocytes.1 The 10/78 antibody competes with the 3.2.3 antibody for binding to the antigen.1,2 NKR-P1A is a type II integral membrane protein with an extracellular C-type lectin domain, which is an NK cell-activating receptor specific for tumor target cells.3
USAGE
The antibody has been tested by immunofluorescent staining (lesser than or equal to 1 µg/106 cells) with flow cytometric analysis to assure specificity and reactivity. Other applications include immunoprecipitation.1 Since applications vary, each investigator must determine dilutions appropriate for individual use.
| Cat. No. | Description | Clone | Size |
| 22641D | Purified anti-rat NKR-P1A | 10/78 | 0.5 mg |
| 22642D | Biotinylated anti-rat NKR-P1A | 10/78 | 0.5 mg |
| 22644D | FITC labeled anti-rat NKR-P1A | 10/78 | 0.5 mg |
| 22645B | PE labeled anti-rat NKR-P1A | 10/78 | 0.2 mg |
REFERENCES
1. Hünig, T. 1996. Personal communication.
2. Chambers, W.H., N.L. Vujanovic, A.B. DeLeo, M.W. Olszowy, R.B. Herberman, and J.C. Hiserodt. 1989. Monoclonal antibody to a triggering structure expressed on rat natural killer cells and adherent lymphokine-activated killer cells. J. Exp. Med. 169: 1373 - 1389.
3. Ryan, J.C., E.C. Niemi, M.C. Nakamura, and W.E. Seaman. 1995. NKR-P1A is a target-specific receptor that activates natural killer cell cytotoxicity. J. Exp. Med. 181: 1911 - 1915.
PharMingen also supplies a complete line of antibodies to rat cell surface antigens, including:
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