Anti-mouse Panendothelial Cell Antigen, Clone MECA-32

SPECIFICITY

The MECA-32 antibody reacts with a dimer of 50-55-kDa subunits,(1) which is expressed on most or all endothelial cells in the embryonic and adult mouse, with the exception of brain, cardiac, and skeletal muscle.(1,2) Normally in skeletal and cardiac muscle, MECA-32 antigen expression is limited to small arterioles and venules; however, under conditions of inflammation, it can be induced on previously non-expressing vessels in cardiac muscle.(1-4) In the CNS, the panendothelial cell antigen expression is developmentally regulated.(1,5) During embryonic development, the antigen is found on brain vasculature up to day 16 of gestation, after which it disappears.(1) The cessation of MECA-32 antigen expression in the CNS may be associated with the establishment of the blood-brain barrier, which begins on day 16 of gestation.(1) In the adult mouse, inflammation in the CNS can lead to re-expression of the panendothelial cell antigen.(5)

USAGE

This antibody has been tested by immunohistochemical staining of acetone-fixed frozen sections (0.5 - 2.5 µg/ml) to assure specificity and reactivity. Other reported applications include immunoprecipitation(1) and western blot analysis.(1) Since applications vary, each investigator must determine dilutions appropriate for individual use.

For Research Use Only. Not For Diagnostic or Therapeutic Use.

REFERENCES
1. Leppink, D.M., D.K. Bishop, D.D. Sedmak, M.L. Henry, R.M. Ferguson, P.R. Streeter, E.C. Butcher, and C.G. Orosz. 1989. Inducible expression of an endothelial cell antigen on murine myocardial vasculature in association with interstitial cellular infiltration. Transplantation 48: 874 - 877.
2. Hallmann, R., D.N. Mayer, E.L. Berg, R. Broermann, and E.C. Butcher. 1995. Novel mouse endothelial cell surface marker is suppressed during differentiation of the blood brain barrier. Dev. Dyn. 202: 325 - 332.
3. Orosz, C.G., A. van Buskirk, D.D. Sedmak, P. Kincade, K. Miyake, and R.P. Pelletier. 1992. Role of the endothelial adhesion molecule VCAM in murine cardiac allograft rejection. Immunol. Lett. 32: 7 - 12.
4 . Bergese, S.D., R.P. Pelletier, R.G. Ohye, D.A. Vallera, and C.G. Orosz. 1994. Treatment of mice with anti-CD3 mAb induces endothelial vascular cell adhesion molecule-1 expression. Transplantation 57: 711 - 717.
5. Engelhardt, B., F.K. Conley, and E.C. Butcher. 1994. Cell adhesion molecules on vessels during inflammation in the mouse central nervous system. J. Neuroimmunol. 51: 199 - 208.

Cat. No. Description Clone Isotype Size
09921D Purified anti-mouse panendothelial cell antigen MECA-32 Rat IgG2a 0.5mg

PharMingen also supplies a complete line of antibodies to mouse cell surface antigens, including:

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