Anti-mouse Vbeta 8 T-Cell Receptor, Clone F23.1

SPECIFICITY

The F23.1 antibody reacts with the Vbeta 8.1, Vbeta 8.2, and Vbeta 8.3 T-cell receptors (TCR) of mice having the Vbeta b haplotype (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C58, DBA/1, DBA/2).(1,2) These gene loci are deleted in Vbeta a (e.g., C57BR, C57L, SJL, SWR) and Vbeta c (e.g., RIII) mice.(2) Vbeta 8.1 TCR-bearing T cells are clonally eliminated in mice expressing superantigen coded by Mtv-7 (Mls-1a, Mlsa) provirus (e.g., AKR, CBA/J, C58, DBA/2), and activation or elimination of Vbeta 8.1 TCR-expressing T cells by this determinant is partially dependent upon presentation by I-E.(3,4) Mtv-43 and/or exogenous MMTV-SW superantigens also cause incomplete elimination of Vbeta 8.1 TCR-bearing T cells.(4) Soluble and plate-bound F23.(1) antibody activates Vbeta 8 TCR-bearing T cells,(1) soluble antibody blocks cytolysis mediated by Vbeta 8 TCR-bearing CTL's,(1) and in vivo treatment of neonatal mice can arrest intrathymic maturation of Vbeta 8 TCR-bearing T cells.(5)

USAGE

This antibody has been tested by immunofluorescent staining (approx. 1µg/million cells) with flow cytometric analysis to assure specificity and reactivity. For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T-cells. Other reported applications include immunoprecipitation,(1,6) western blot analysis,(6) in vitro stimulation of T cells expressing Vbeta 8.1 or 8.2 TCR,(1) in vitro blocking of cytolysis,(1) and in vivo inhibition of thymocyte maturation.(5) Since applications vary, each investigator must determine dilutions appropriate for individual use.

For Research Use Only. Not For Diagnostic or Therapeutic Use.

REFERENCES
1. Staerz, U.D., H.-G. Rammensee, J.D. Benedetto, and M.J. Bevan. 1985. Characterization of a murine monoclonal antibody specific for an allotypic determinant on T cell antigen receptor. J. Immunol. 134: 3994 - 4000.
2. Behlke, M.A., T.J. Henkel, S.J. Anderson, N.C. Lan, L. Hood, V.L. Braciale, T.J. Braciale, and D.Y. Loh. 1987. Expression of a murine polyclonal T cell receptor marker correlates with the use of specific members of the Vbeta8 gene segment subfamily. J. Exp. Med. 165: 257 - 262.
3. Kappler, J.W., U. Staerz, J. White, and P.C. Marrack. 1988. Self-tolerance eliminates T cells specific for Mls-modified products of the major histocompatibility complex. Nature 332: 35 - 40.
4 . Hodes, R.J., and R. Abe. 1996. Mouse endogenous superantigens: Mls and Mls-like determinants encoded by mouse retroviruses. In: Current Protocols in Immunology. J.E. Coligan, A.M. Kruisbeek, D.H. Margulies, E.M. Shevach, and W. Strober, eds. John Wiley & Sons, New York, NY, pp. A.1F.1 - A.1F.5.
5. Kyewski, B.A., V. Schirrmacher, and J.P. Allison. 1989. Antibodies against the T cell receptor/CD3 complex interfere with distinct intra-thymic cell-cell interactions in vivo: correlation with arrest of T cell differentiation. Eur. J. Immunol. 19: 857 - 863.
6. Saint-Ruf, C., K. Ungewiss, M. Groettrup, L. Bruno, H.J. Fehling, and H. von Boehmer. 1994. Analysis and expression of a cloned pre-T cell receptor gene. Science 266: 1208 - 1212.

Cat. No. Description Clone Isotype Size
09951C Purified anti-mouse Vbeta 8 TCR F23.1 Mouse IgG2a 0.25mg
09952C Biotinylated anti-mouse Vbeta 8 TCR F23.1 Mouse IgG2a 0.25mg
09954C FITC labeled anti-mouse Vbeta 8 TCR F23.1 Mouse IgG2a 0.1mg

PharMingen also supplies a complete line of antibodies to mouse T Cell Receptors and MHC molecules.

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