Quantitative determination of LDL receptor expression on monocytes A. Sample Preparation 1. Cell Isolation 2. Cell Culture 3. Cell Harvest 4. Cell Staining B. Measurement Set a live gate on monocytes during aquisition C. Data Evaluation Determine the LDL receptor activity (= mean fluorescence = amount of LDL receptors) Report your results

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu