Test for the Quantification of the Cytotoxic Activity of Natural Killer (NK) Cells A. Sample Preparation 1. Isolation of Effector Cells 2. Thawing of Target Cells 3. NK Functional Test B. Measurement Set a live gate on green fluorescent target cells during acquisition C. Data Evaluation Determine the percentage of dead target cells ( = fluorescence 3 positive events) Report your results

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu