PFA fixed cells

Aleta Knowles (A.Knowles@vetp.usyd.edu.au)
Fri, 2 Aug 1996 10:57:59 +1100

Hello everyone,
I'm looking at phagocytosis and oxidative burst in porcine neutrophils.
I plan on performing a simultaneous assay using FITC-zymosan and
hydroethidium, and will be processing lots of samples by flow cytometry.
I would like to fix the cells in PFA after phagocytosis - will fixation
affect the resulting flourescence of EtBr produced during the oxidative
burst?
Thanks
Aleta 

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Aleta Knowles


Department of Veterinary Pathology                 Phone: 61 2 351 7651
The University of Sydney                           Fax:  61 2 351 7348
NSW, 2006
AUSTRALIA