> I'm looking at phagocytosis and oxidative burst in porcine neutrophils.
> I plan on performing a simultaneous assay using FITC-zymosan and
> hydroethidium, and will be processing lots of samples by flow cytometry.
> I would like to fix the cells in PFA after phagocytosis - will fixation
> affect the resulting flourescence of EtBr produced during the oxidative
> burst?
You'll probably find the fixation allows the EtBr to leak out, so in time,
all cells will become equally EtBr-stained.
\ / < Flow Systems Laboratory
Frank Battye \__/ <<<<< The Walter & Eliza Hall Institute
Robyn Muir ----------!!<<<<<<<< Voice: 61_3_9345 2540
Dora Kaminaris /!!\ <<<<< Fax: 61_3_9347 0852
o !! \ < IN:: "facs_copy@wehi.edu.au"