apoptosis in vivo
ZBIGNIEW DARZYNKIEWICZ (darzynk@nymc.edu)
Sat, 03 Aug 1996 11:27:06 -0500
Apoptosis in tissue sections as well as in fixed cytocentrifuged- or
smeared- cells can be detected by labeling DNA strand breaks with
fluorochrome-tagged deoxynucleotides, using exogenous terminal
transferase (e.g. Cell Proliferation, 28: 571, 1995). Since the cells
are fixed with with formaldehyde (1 %, 15 min or more) and
subsequently permeabilized by treatment with ethanol or detergent,
the method is compatible with simultaneous immunocytochemical
detection (using another color fluorochrome conjugated antibody) of
any protein whose epitope remains unchanged by this fixation. Most
proteins remain reactive with antibodies following such treatment.
Zbigniew Darzynkiewicz