Strategy seems OK, FITC is the commonest fluorochrome conjugated to
antibodies. I assume when you say CY5 you mean PE/CY5 CD4 , CY5 needs a red
laser.
Simon
Simon Monard
FACS Lab Manager
Aaron Diamond Center for AIDS Research
455 First Avenue
New York
NY 10016
Ph (212) 448 5141
FX (212) 725 1126