I am looking for a positive control for inducing apoptosis in
cultivated macrophages/microglial cells. I did some Annexin/PI stains, however, Colchicin
and growth-factor-withdrawl does not look very promising to me. (It just increases the
number of Annexin/PI-stained "necrotic" cells).
I would highly appreciate any hint.
Joachim v. Zahn
Max-Delbrueck-Centrum
Cellular Neurosciences
Robert-Roessle-Str. 10
13122 Berlin
Germany
jzahn@orion.rz.mdc-berlin.de