Re: sperm and flow cytometry

Geoffrey W Osborne (gwo215@cscgpo.anu.edu.au)
Fri, 30 Apr 93 09:46:22 EST

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Hi,
At one time we were doing a lot of bovine sperm sorting, all X-Y stuff.
We had to design and make our own nozzle holder such that the core stream can
be moved to orientate the sperm, and use a wedge shaped end on the core stream
injection needle to try to get them going down head first. I know that native
australain mice sperm have a characteristic hooked shaped tail, which in light
of the above may cause stability/orientation problems, but this may not be the
case for laboratory mice or wild US mice!
The only other advice I can offer is be careful what sheath fluid you
use as this seemed to make a real different to the "viability" of the sorted
sperm. Hope this helps,

Geoff Osborne
Flow Cytometry (FACS LAB)
John Curtin School of Medical Research,
Australian National University,
CANBERRA, AUSTRALIA.

Email: Geoff.Osborne@anu.edu.au

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