Re: S phase in situ

Anna Porwit-MacDonald (anpo@mb.ks.se)
Tue, 23 Sep 1997 14:42:50 +0200

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Hi!
You can get quite near to counting % of S-phase cells using
immunohistochemistry (ABC method with microwave) and a monoclonal Ab to
human Cyclin A (NCL-Cyclin A from Novocastra Lab.,UK).
We have been using it for a while now and the stainings work well even on
the old archival material.
With best wishes
Anna

Anna Porwit-MacDonald, MD, PhD
Haematopathology Lab.,
Karolinska Hospital
Stockholm, Sweden

>just a quick scan to ask if there is a marker (monoclonal antibody)
>available which would allow the quantitation of %S-phase cells in a B5
>fixed lymph node (formalin fixed paraffin embedded).
>
>thank you
>
>Dr. MRG O'Gorman
>Assis. Prof. Pediatrics
>Northwestern University
>Director Diag. Immunol and Flow Cytometry Labs.
>Children's Memorial Hospital
>Chicago IL
>Ph. 773 880 3070
>Fax 773 880 3739
>
>
>
>

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