RE: Cell killing and CD markers

Scott R. Burger (burge009@gold.tc.umn.edu)
Wed, 15 Oct 1997 12:33:56

> We are attempting to produce a population of "dead" WBC to spike into
> a live dead assay and need to find a way to kill the cells without
> destroying the CD14 marker.

Dear Dr. Koratich:

We've used a brief (10 minute) incubation at 65oC to kill hematopoietic
progenitor cells for a similar purpose -- generating a standard curve of
viable/dead cells for assay validation.

I've never looked at expression of CD14 on these cells, but the method does
kill cells while leaving them structurally intact.

Scott

-----------------------------------------------
Scott R. Burger, M.D.
Medical Director, Cell Therapy Clinical Laboratory
Department of Laboratory Medicine and Pathology
University of Minnesota
burge009@gold.tc.umn.edu
612-626-4919
612-624-5411 (Fax)