RE: Cell killing and CD markers
Rafael Nunez (rafaeln@vetvir.unizh.ch)
Thu, 16 Oct 1997 09:30:14 +0100
Dear Dr Scott
I am very much interested in your experiments with heating the cells at 65
degrees.
I used to heat Dendritic cells at 56 degrees for 30 minutes in order to
abolish proliferation.
In your experiments do you test if the structural antigens on the surface
remain intact or in some way are they altered?, Have you look if your dead
cells are in some stage of apoptosis?.
Regards
Rafael
>> We are attempting to produce a population of "dead" WBC to spike into
>> a live dead assay and need to find a way to kill the cells without
>> destroying the CD14 marker.
>
>Dear Dr. Koratich:
>
>We've used a brief (10 minute) incubation at 65oC to kill hematopoietic
>progenitor cells for a similar purpose -- generating a standard curve of
>viable/dead cells for assay validation.
>
>I've never looked at expression of CD14 on these cells, but the method does
>kill cells while leaving them structurally intact.
>
>Scott
>
>
>
>-----------------------------------------------
>Scott R. Burger, M.D.
>Medical Director, Cell Therapy Clinical Laboratory
>Department of Laboratory Medicine and Pathology
>University of Minnesota
>burge009@gold.tc.umn.edu
>612-626-4919
>612-624-5411 (Fax)
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