Immunophenotyping of rat spleen

Miller, Judith E. (judy_miller@merck.com)
Mon, 20 Oct 1997 11:54:41 -0400

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I am performing immunofluorescent staining of rat spleen cells. I am
using a stomacher to prepare single cell suspensions and ammonium
chloride to lyse RBCs. I have found that the cell viability and
antibody binding is affected. I have attempted to analyze without RBC
lysing, but I have not been able to eliminate the RBCs electronically.

Does anyone have a procedure for CD45 antibody labeling of rat spleen
cells for gating of lymphocyte subsets and excluding RBCs? I am also
interested in other procedures for processing rat spleen, RBC lysing, or
flow analysis without lysing. Thanks!

Judy Miller
Merck & Co., Inc.
Phone: 215-652-3127
Fax: 215-652-7758
E-mail: millerj@merck.com

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