CD34 selection and DNAse
Jan Nelson (j.nelson@auckland.ac.nz)
Tue, 21 Oct 1997 12:30:31 +1200
We found when thawing pilot vials from PB and bone marrow samples or
diluting an infusion sample we could reduce clumping by adding preservative
free heparin to the diluent at 10 u/ml. The vial is thawed at 37C and
diluted in 9 volumes of IMDM or saline.This does allow you to dilute in
IMDM which would cause clumping otherwise. We don't add DNAse.
Jan
Jan Nelson
Molecular Haematology
Department of Molecular Medicine
The University of Auckland
Phone: (+64)(9) 373-7599 ext 6381
Fax: (+64)(9) 373-7292