I haven't flowed pig WBC, but cow, cat, sheep, and hamster. If you don't
want to Ficoll your cells (dilute your blood 1:2 or 1:3), you can try to use
an NH4Cl-based lysing solution.
An easy to prepare is: 8.99 g/L NH4Cl, 1g/L KHCO3, 37 mg/L tetrasodium EDTA,
pH 7,3. Add 7 ml of lysing solution to 500 microliter of your blood, mix
gently and when RBC will are lysed spin for 7 minutes. Wash 2x in saline or
PBS and resuspend in your staining media. I usually stain 10 samples
starting from 500 microliters of blood (cow PMN phenotyping).
Hope this help
Mara
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Mara Rocchi, DVM, Ph.D.
International Livestock Research Institute
Nairobi, Kenya
Fax: 00254-2-631499
e-mail: M.Rocchi@cgnet.com