cell sorting

DoranC@aol.com
Thu, 23 Oct 1997 17:07:44 -0400 (EDT)

I have recently been trained on the FACS Vantage and was told that while
sorting cells, the abort rate should not be greater than 10% of the event
rate (sys. threshold). My problem is that the abort rate is running approx.
20 - 30%. I acquire events usually around 2000, and most often sort
normal-R, 3 drops. I have tried to alter the concentration, slow down the
rate, and even filter the sample thru a filter cap. Any ideas?