cy3 positive control using beads

Hector Nolla (hector@soest.hawaii.edu)
Thu, 23 Oct 1997 13:57:55 -1000 (HST)

---------- Forwarded message ----------
Date: Thu, 23 Oct 1997 12:11:36 -1000
From: Markus Karner <mkarner@soest.hawaii.edu>
To: cyto-inbox
Subject: cy3

Hello all, This is a question from one of our users. Thanks in advance.

Here it is:

---
We are trying to apply flow cytometry to analysis of marine bacteria after
FISH using Cy3 Dye labelled oligonucleotides. Is there an easy way for a
positive control, for example, using beads to which the oligonucleotide would
adsorb, so that they could be counted? Does anybody know of beads to which
DNA-oligonucleotides would readily adsorb? Is an argon 
laser tuned to emit at 514nm  suitable for Cy3 excitation?

---


Thanks


Markus

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