Estimation of Fetal Maternal Haemorrhage by flow
Drew Randall (drandall@cyllene.uwa.edu.au)
Fri, 24 Oct 1997 21:42:39 +0800
We are looking at performing quantitation of D positive cells (FMH) by =
flow cytometry. I have been trialing Fluro-D (FITC labelled, CSL =
product) using the method as recommended by the manufacturer. So far I =
have found that my standard curve results (O Neg blood spiked with O Pos =
blood) don't always correlate as close as I would expect/like. Is this =
due to poor technique or have other operators found similar results. So =
far, I have been able to detect low levels of D positive cells with =
minimal background. However, I would appreciate any comments from other =
users about potential pitfalls, correlation to Kleihauer results, best =
method of reporting (% or mls of D cells) and any other general hints.
Thanks to all replies,
Wanda