Re: Oregon Green 488

Mahesh Bhalgat (MaheshBhalgat@probes.com)
Fri, 24 Oct 1997 09:44:24 -0700

As far as photostability is concerned, I would suggest looking at the
back cover of BioProbes 23 which shows a good comparison between
Fluorescein and Oregon Green 514. The photostability is dependent upon
the medium in which mounting is done. Both Oregon Green 488 and Oregon
Green 514 show good photostability in Prolong, SlowFade, and CytoSeal.
In PBS however, OG 514 shows better photostability than OG 488.
As a matter of fact, the new dye Alexa 488 is not only brighter than OG
488 and Fluorescein but is also more photostable than those.

Mahesh K. Bhalgat, Ph.D.
Staff Scientist/Flow Cytometry Products Manager
Molecular Probes, Inc.
OREGON

Elaine Kunze wrote:

> Does anyone have any experience with Oregon Green 488? Is it a lot
> more
> photostable than Fitc for microscopic applications? Is it worth the
> $$$.
>
> ************************************
> Elaine Kunze
> Flow Cytometry......Image Analysis
> The Biotechnology Institute for Research and Education
> 8B Althouse Lab
> Penn State University
> University Park, PA 16802
> (814) 863-2762