Does anybody of you have experience of using the TUNEL technique to test
apoptosis in mouse thymocytes directly ex vivo (no incubation in culture) ?
I'm just curious if one can detect the apopotosis of double positive
thymocytes directly, or if a incubation in culture is necessary to see
anything...
I know that this subject has been discussed before, but it's very hard to
search the archive from here, as the network connections are terribly
jammed in the moment...
Thanks a lot again,
Matthias
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Matthias Haury Flowcytometry Dept Immunology Institut Pasteur
mhaury@pasteur.fr Tel: 33 (01) 40 61 31 29 Fax: 33 (01) 45 68 86 39
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