distinguishing specific from non-specific binding. I have started to
tinker with some anti-IgG-Fc (Fab) - but am experiencing horrific
backgrounds at first attempts - so any tips/tricks on blocking, pre- or
post -stain fix, whether whole blood techniques are common, and if so -
when to lyse. What are people using in detection of leucocyte or platele=
t
antibodies, flow crossmatches, etc?
Robin Barclay
Blood Transfusion Service
Edinburgh