Jet Velocity
Vincent Falco (vincent.falco@es.nemc.org)
Thu, 6 Nov 97 7:57:56 EST
To All:
Way back when I was taught that the jet velocity out of a nozzle for sorting
was optimal at 10m/s.This is a jet in air system,no quartz flow cell.The
method to calculate this jet velocity from volume equations for a cylinder
are given in Howard Shapiros Practical Flow Cytometry.While taking these
measurements may be crude,the overall approximation is good.What I would like
to know is,Does anyone check the velocity out of the nozzle?If so, what do
you set your sheath pressure at to get this velocity?What is the velocity you
use for your system,either BD, COULTER, CYTOMATION, OR HOMEGROWN. I am
talking the standard type sorters.Not your TSO from BD OR OTHER HIGH SPEED
SYSTEM.