Although I cannot furnish you with a reference, I recall reading some
literature in the past which described the amount of DNA in human cells in
mass terms was 7.5-7.9 picograms. Activated lymphocytes or proliferative
cells (ie, G1 as opposed to G0 cells) within tissues were said to contain
~8.3 pg per nucleus - which may explain the near diploid peak seen by some
investigators in the analysis of PB and some solid tissues. Trout red blood
cells used for control purposes contain ~5.0 pg/nucleus whilst the DNA mass
in chick red blood cells escapes me. I have remembered these figures for my
research interests in cell ploidy and proliferation, but would not know
where or if I have the source of this information now. If someone does give
you a reference I would be pleased to know of it.
Regards,
Jeanette.
At 07:22 PM 4/11/97 +0000, you wrote:
>
>Dear all
>
>Another question
>
>In a survey of a cultivated plant for local species/type/variant diversity, we
>intend to use normal human lymphocytes as a reference standard for DNA
>nuclear content.
>
>What we need to know is; please can anyone tell us the DNA content of
>normal diploid human lymphocytes in mass terms per cell?
>
>Alternatively does anyone have any ideas for a reference text where we may
>find this information?
>
>Failing that are there any better/cheaper/more appropriate standards which we
>could use?
>
>Thanks.
>
>DR Lloyd
>University of Birmingham
>England
>
>
>Regards
>
>D
>
>t; +44 (0)121 414 5264
>f; +44 (0)121 414 5324
>e; D.R.Lloyd@bham.ac.uk
>
>
_______________________________________________________________
Jeanette Drew
Department of Obstetrics & Gynaecology
University of Western Australia / King Edward Memorial Hospital
SUBIACO WA 6009 AUSTRALIA
Tel: 61-8-9340-2768 ,-_|\
Pager: 61-8-9485-6570 / \
Fax: 61-8-9340-2636 JD -> *_,-._/
Email: jdrew@cyllene.uwa.edu.au v
_______________________________________________________________