stain all cell/bacteria

Georgia M. Farris (farris@telomere.lanl.gov)
Fri, 25 Oct 1996 10:19:14 -0600

I am looking for advise on a stain/dye that will penetrate cells
(specifically bacteria) or bind with the surface membrane and not have to be
washed off before acquisition. The stain/dye must excite with a 488nm laser
and need either no fixation or a fixative that is mixed with the dye and
does not need washing before acquisition. Even if your experience is only
with mammalian cells I would be willing to try a recommended technique in
our lab with the bacteria.

Please send advise to farris@telomere.lanl.gov

Thank you, Georgia M. Farris
farris@telomere.lanl.gov