OptEIA™ Sets for Cytokine ELISA Development

This new sandwich ELISA system provides an alternative for researchers who want faster assays that are also suitable for serum cytokine analysis.

OptEIA™ Sets have been specially formulated for analysis of serum, plasma, and cell culture supernatant samples.

The OptEIA™ Set contains matched pre-titered reagents. Each lot's performance is optimized after preparation and testing as working solutions. Lot-specific instructions and optimal concentrations are provided. No reagent titration is necessary. Each set provides the following reagents:

• Capture antibody
• Horseradish peroxidase or biotin conjugated detecting antibodies
• Avidin-horseradish peroxidase (for Sets with Biotin conjugates)
• Recombinant Standard

Sets Advantages:

• Specially formulated and validated for serum, plasma, and cell culture supernatant cytokine quantitation
• Lot-specific reagent concentrations and assay data provided
• Fraction of kit price-per-plate
• Sufficient reagents for 40 plates of assays (when following suggested protocols)
• Pre-titered reagents; no assay optimization required
• Outstanding value

OptEIA™ Sets:

Human

• IL-4
• IL-12 (p70)
• IL-5
• GM-CSF
• IL-6
• IFN-gamma
• IL-8 (inquire)
• Soluble Fas (NEW!)
• IL-10
• TNF-alpha
• IL-12 (p40)

Mouse (please inquire for availability)

• IL-3
• IL-12 (p70)
• IL-4
• IgE
• IL-5
• MCP-1
• IL-6
• TCA3
• IL-10
• TNF-alpha

For Research Use Only. Not For Diagnostic or Therapeutic Use.

PharMingen also supplies a number of additional reagents for cytokine and chemokine analysis, including:

• Paired antibodies and recombinant proteins (sold separately) for ELISA quantitation
• Antibodies to cytokine and chemokine receptors
• RiboQuant™ RNase Protection Assay featuring Multi-Probe template sets for cytokine mRNA detection and quantitation

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